WEE1, Active (W01-11G)
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Description : Recombinant full-length human WEE1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species : Human
Tag : N Terminal GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence : Full length
Genbank Number :NM_003390
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~115 kDa
Gene Aliases : WEE1hu, WEE1A
Scientific Background :WEE1 is a nuclear protein kinase that regulates G2/M transition in response to DNA damage. It catalyzes an inhibitory tyrosine phosphorylation of cyclin-dependent kinase 1-bound cyclin B resulting in G2 cell cycle arrest (1). WEE1 also maintains the proper timing of cell division through phosphorylation of tyrosine15 residues of cyclin-dependent kinases 1 and 2, thereby modulating the activity of both kinases and controlling entry into mitosis and DNA replication during S phase (2).
References :
1. Heald, R. et.al: Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated cdc2 kinase. Cell 74: 463-474, 1993.
2. Parker, L. L. et.al: Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase. Science 257: 1955-1957, 1992.
Product Sheets (By Lot #) :
Research Areas :Ser/Thr Kinases, JAK/STAT Pathway, Inflammation, Cardiovascular Disease, Cancer, Cancer, Inflammation, Cardiovascular Disease, JAK/STAT Pathway, Cytoplasmic Tyrosine Kinases