USP8, Active (U508-381H)
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Description : Recombinant human USP8 (630-end) was expressed in E. coli cells using an N-terminal His tag.
Species : Human
Tag : His tag
Expression System : E.coli
Sequence : 630-end
Genbank Number : NM_005154
Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet. 
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Activity Assay, Western Blot
Molecular Weight : 55 kDa
Gene Aliases : KIAA0055, UBPY
Scientific Background : USP8 (ubiquitin carboxyl-terminal hydrolase 8) is a member of the ubiquitin-specific processing protease family of proteins and is involved in the regulation of endosomal ubiquitin processes for endosome morphology, cargo sorting and membrane trafficking at the early endosome stage. USP8 deubiquitinates EPS15 and regulates EGFR degradation and MAPK signaling via removing conjugated ubiquitin from EGFR (1). USP8 also interacts with spermatid ESCRT-0 and microtubule structures in mouse species, suggesting that it may be involved in acrosome biogenesis (2).
References :
1. Berlin, I. et al: Regulation of epidermal growth factor receptor ubiquitination and trafficking by the USP8·STAM complex. Journal of biological chemistry 285(45), 34909-21, 2010.
2. Giovanna,B. et al. USP8, a Regulator of Endosomal Sorting, Is Involved in Mouse Acrosome Biogenesis Through Interaction with the Spermatid ESCRT-0 Complex and Microtubules, Biology of Reproduction, 82(5), 930–939, 2010.
Product Sheets: (by Lot #):
Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder