CRBN/DDB1 Complex(U274-34H)
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Description :Recombinant full-length human CRBN and DDB1 were expressed by baculovirus in Sf9 insect cells using N-terminal His-tags for both proteins.
Species :Human
Tag :HIS
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_016302
Genbank Number 2 : NM_001923
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Alternative Name(s) :CRBN: CRBN; MRT2; MRT2A; protein cereblon
DDB1: DDB1; DDBA; UV-DDB1; WHIKERS; XAP1; XPCE; XPE; DNA damage-binding protein 1
Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :CRBN (Cereblon) and DDB1 (DNA damage-binding protein 1) are proteins that play crucial roles in various cellular processes, including DNA repair, cell cycle regulation, and protein degradation. CRBN is a substrate receptor protein, while DDB1 acts as an adaptor protein within the Cullin-RING ubiquitin ligase (CRL) E3 ligase complex. This complex plays a critical role in the ubiquitin proteasome pathway, which controls the targeted degradation of specific proteins. Understanding the intricate roles of CRBN and DDB1 in cellular processes is crucial for deciphering their potential as therapeutic targets and advancing our knowledge of various diseases, particularly cancer, where dysregulation of these proteins has been observed.
References :1. Chamberlain PP, Lopez-Girona A, Miller K, et al. Structure of the human Cereblon-DDB1-lenalidomide complex reveals basis for responsiveness to thalidomide analogs. Nat Struct Mol Biol. 2014;21(9):803-809. doi:10.1038/nsmb.2874
2. Ito T, et al. (2010). Identification of a primary target of thalidomide teratogenicity. Science, 327(5971), 1345-1350.
3. Sugasawa K, et al. (2005). A multistep damage recognition mechanism for global genomic nucleotide excision repair. Genes & Development, 19(17), 2192-2203.
Molecular Weight :CRBN observed MW ~58 kDa.
CRBN calculated MW ~51 kDa.
DDB1 observed MW ~128 kDa.
DDB1 calculated MW ~131 kDa.
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