UBE2D1 (UBCH5A), Active(U213-380H)
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Description :Recombinant full-length human UBE2D1 was expressed in E. coli cells using an N-terminal His tag.
Species :Human
Tag :His tag
Expression System:E.coli
Sequence :Full length
Genbank Number :NM_003338
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Ubiquitin Enzyme Assay, Western Blot
Molecular Weight :17 kDa
Gene Aliases :E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A
Scientific Background :UBE2D1 (UBCH5A) or ubiquitin-conjugating enzyme E2D1 is a member of the E2 ubiquitin-conjugating enzyme family which is closely related to a stimulator of iron transport (SFT). UBE2D1 is significantly upregulated in livers of iron-overloaded patients with hereditary hemochromatosis. UBE2D1 also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF-1α by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
References :
1. Mondal, S. et al: A bioluminescent assay for monitoring conjugation of ubiquitin and ubiquitin-like proteins. Anal. Biochem. 510: 41-51, 2016
2. Gehrke, S.G. et al: UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis. Blood 101: 3288-3293, 2003
3. Scheffner, M. et al: Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53. Proc. Nat. Acad. Sci. 91: 8797-8801, 199
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Research Areas :Cancer, Cell Cycle, Neurobiology