NAE1/UBA3 Complex, Active (U203-380G)
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Description :Recombinant human NAE1 (2-end) and full-length human UBA3 were expressed by baculovirus in Sf9 insect cells.
Species :Human
Tag : HIS/GST tag
Expression System: Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number : NM_003905 and BC022853
Specific Activity : The specific activity of NAE1/UBA3 was determined to be 6.4 nmol/min/mg as per activity assay protocol
Purity : The purity of NAE1/UBA3 was determined to be >70% by densitometry.
NAE1 approx. MW 65 kDa.
UBA3 approx. MW 73 kDa.
Storage, Stability, and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Ubiquitin Enzyme Assay, Western Blot
Molecular Weight : NAE1 approx. MW 65 kDa. UBA3 approx. MW 73 kDa.
Gene Aliases : NAE1 - APPBP1
UBA3 - UBE1C, UBA3, hUBA3
Scientific Background : NAE1/UBA3 is a heterodimeric E1 (Ub-activating)–like complex that activates NEDD8. In the presence of ATP, NAE1/UBA3 adenylates and activates NEDD8 yielding NEDD8-UBA3 thioester and free AMP. Activated NEDD8 is then transferred to UBE2M or UBE2F through transthiolyation reaction. Finally, substrate specific-E3 ligase, such as RBX1/2 or DCN1, transfers NEDD8 from UBE2M or UBE2F to a lysine residue in its target protein (1,2). NEDD8 pathway plays an important role in cell cycle progression and survival (3).
References :
- Zhou, L. et al: Neddylation: a novel modulator of the tumor microenvironment. Mol Cancer. 18: 77, 2019.
- Gong, L. et al: Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. J Biol Chem. 23 (274):12036-42, 1999.
- Soucy, T.A. et al: The NEDD8 conjugation pathway and its relevance in cancer biology and therapy. Genes Cancer. 1: 708-716, 2010.
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Research Areas :Cancer, Cell Cycle, Neurobiology