Tau K18 (P61L) Pre-Formed Fibril(T18-58GN)

Tau K18 (P61L) Pre-Formed Fibril(T18-58GN)

  • $455.00


Description :Recombinant tag-free human Tau K18 (P61L) Protein Monomer was expressed in E. coli cells.

Species :

Tag :TAG-Free

Expression System:E.coli

Sequence :

Genbank Number :P10636

Alternative Names :Microtubule-associated protein tau; MAPT; MAP; Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1; Isoform 4

Formulation :Recombinant protein stored in 10mM HEPES, pH 7.4, 100mM NaCl.

Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background :Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). K18 is a truncated form of human tau containing only the 4 microtubule binding repeats (5). P301L (PL) is a mutation where proline is replaced by leucine at codon 301 of tau, and has been linked to frontotemporal dementia (6).

References :1. www.alz.org/alzheimers-dementia/facts-figures
2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907)
3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497.
4. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18.
5. Guo, J. and Lee, M.Y. (2013). FEBS Lett. 587(6): 717-723.
6. Alberici, A. et al. (2004). J Neurol Neurosurg Psychiatry. 75:1607-1610.

Sample Data :SDS-PAGE of ~15 kDa Human Tau Protein K18 P301L Pre-Formed Fibrils (Cat# T18-58GN). Lane 1: MW Ladder. Lane 2: Tau Protein Pre-Formed Fibrils (Cat# T18-58GN).

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Research Areas :Neurobiology