Single-stranded DNA binding protein (SS01-C501H)
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Description : Recombinant full-length E. coli Single-stranded DNA binding protein was expressed in E. coli with an N-terminal His tag.
Species :Human
Tag :HIS tag
Expression System: E.Coli
Sequence : Full length
Genbank Number : J01704
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight :~20 kDa
Gene Aliases : ssb, exrB, lexC,
Scientific Background : Single-stranded DNA binding protein (SSB) in E. coli is involved in DNA replication, repair, and recombination (1). SSB enhances helix destabilization by DNA helicases thus preventing reannealing of single-stranded DNA and nuclease digestion during DNA replication (1). E. coli SSB is required for methyl-directed mismatch repair, induction of the SOS response, and recombinational repair (1). SSB interacts with DNA helicase RecQ of the RecF-recombination pathway and stimulates helicase activity (2). SSB also stabilizes replication origins, primosome assembly, plays a role in primosome assembly, enhances replication fidelity and polymerase processivity, and promotes polymerase binding to template DNA (1,2).
References :
- Meyer, R. R., Laine, P. S. The single-stranded DNA-binding protein of Escherichia coli. Microbiol Rev. 1990, 54(4): 342-380.
- Shereda, R. D. et al. SSB as an organizer/mobilizer of genome maintenance complexes. Crit Rev Biochem Mol Biol. 2008, 43(5): 289-318.
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Research Areas :Cancer, Neurobiology, Inflammation, Cell Cycle, Invasion/Metastasis, Cytoplasmic Tyrosine Kinases, Cancer, Neurobiology, Inflammation, Cell Cycle, Invasion/Metastasis, Cytoplasmic Tyrosine Kinases