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Anti-STAT3(S54-63BR)

Anti-STAT3(S54-63BR)

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Description :Rabbit Polyclonal Antibody

Specificity :Recognizes the STAT3 protein

Cited Applications :WB, IP

Cross Reactivity :Western blot of human STAT3

STAT3 from other species may also be detectable

Host :Rabbit

Immunogen :Synthetic peptide corresponding to amino acids 725-729 of human STAT3

Purification :Affinity chromatography

Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative immunoprecipitation with 2 μg of Anti-STAT3 using 500 μg of HeLa, HEK-293T, Jurkat, 3T6, and THP1 cell lysate.

Sample Data :Representative western blot with Anti-STAT3 (1:1000) using 20 ng of human recombinant STAT1β, STAT2, STAT3, STAT4, STAT5, and STAT6.

Sample Data :Representative western blot with Anti-STAT3 (1:1000) using 30 μg of HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysate.

Scientific Background :STAT3 is a member of the signal transducers and activators of transcription (STAT) family of proteins that carry out a dual function: signal transduction and activation of transcription. STAT3 is widely expressed and becomes activated through phosphorylation on tyrosine as a DNA binding protein in response to a variety of stimuli such as EGF, IL-6, PDGF, IL-2 and G-CSF (1). This phosphoprotein forms homodimers as well as heterodimers with other members of the STAT family and translocate to the nucleus in order to modulate the transcription of various genes (2).

References :
1. Zhong, Z. et al: Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6. Science. 1994 Apr 1;264(5155):95-8.

2. Tian, S. S. et al: Rapid activation of the STAT3 transcription factor by granulocyte colony-stimulating factor. Blood. 1994 Sep 15;84(6):1760.

Product Sheets (By Lot #) :

H283-8.pdf

Research Areas :Cancer, Inflammation, ERK/MAPK Pathway, JAK/STAT Pathway, Apoptosis/Autophagy, Cancer, Inflammation, ERK/MAPK Pathway, JAK/STAT Pathway, Apoptosis/Autophagy