Alpha Synuclein (A53T) Protein(S22-36N)
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Description :Recombinant full-length tag-free human Alpha-synuclein (A53T) mutant Protein was expressed in E. coli cells.
Species :Human
Tag :TAG-Free
Expression System:E.coli
Sequence :Full Length
Genbank Number :NP_000336.1
Alternative Names :A53T mutant alpha synuclein; A53T mutated SNCA; Non-A beta component of AD amyloid protein; Non-A4 component of amyloid precursor protein
Formulation :Recombinant protein stored in PBS, pH 7.4.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). The A53T mutation is a missense point mutation where alanine is replaced by threonine at the 53rd amino acid. This mutation has been linked to early-onset Parkinson's Disease (7) and increased rates of alpha synuclein fibrillization (8).
References :1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013).
2. Zhang L., et al. (2008) Brain Res. 1244: 40-52.
3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117.
4. Kokhan V.S., Afanasyeva M.A., Van’kin G. (2012) Behav. Brain. Res. 231(1): 226-230.
5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840.
6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.
7. Polymeropoulos, M. H. (1998). Science. 276(5321), 2045–2047
8. Conway, K.E., et al. (1998). Nat Med. 4(11):1318-20
Sample Data :SDS-PAGE of ~14 kDa Alpha Synuclein (A53T) Protein (Cat# S22-36N). 
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