PKC theta, Active(P74-10G)
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Description :Recombinant full-length human PKCtheta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_006257
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~110 kDa
Gene Aliases :PRKCQ, PRKCT, MGC126514, MGC141919, nPKC-theta
Scientific Background :Protein Kinase C, theta (PKCθ) is important component in the intracellular signaling cascade (1). Recent studies have suggested that local accumulation of fat metabolites inside skeletal muscle may activate a serine kinase cascade involving PKC-theta leading to defects in insulin signaling and glucose transport in skeletal muscle (2). Insulin resistance plays a primary role in the development of type 2 diabetes and may be related to alterations in fat metabolism. PKC-theta is a crucial component mediating fat-induced insulin resistance in skeletal muscle and is a potential therapeutic target for the treatment of type 2 diabetes (2).
References :
1. Manicassamy, S. and Sun, Z. The critical role of protein kinase C-theta in Fas/Fas ligand-mediated apoptosis. J. Immunol. 2007;178(1):312-9.
2. Kim, J K. et al: PKC-theta knockout mice are protected from fat-induced insulin resistance. J. Clin. Invest. 2004;114(6):823-7.
Product Sheets (By Lot #) :
Research Areas :Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, NfkB Pathway, PKA/PKC Pathway, Apoptosis/Autophagy, Ser/Thr Kinases, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, NfkB Pathway, PKA/PKC Pathway, Apoptosis/Autophagy, Ser/Thr Kinases