AMPK (A1/B1/G1), Active (P47-110GH)

AMPK (A1/B1/G1), Active (P47-110GH)

  • $435.00


Description: Recombinant full-length human AMPK (a combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using N-terminal GST and C-terminal His tags.

Species :Human

Tag :GST/HIS tags

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number 1 :NM_006251

Genbank Number 2 :NM_006253

Genbank Number 3 :NM_002733

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at the recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight:~94-100kDa (A1), ~64kDa (B1), ~68kDa (G1)

Gene Aliases: Subunit A1: PRKAA1, MGC33776, MGC57364
Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785
Subunit G1: PRKAG1, AMPKG, MGC8666

Scientific Background :AMPK is a heterotrimer protein kinase consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status (1). In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol (2).

References :
1. Minokoshi, Y. et al:AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus. Nature 428: 569-574, 2004.

2. Hardie, D G. et al: The AMP-activated protein kinase--fuel gauge of the mammalian cell Eur J Biochem. 1997 Jun 1;246(2):259-73.

Product Sheets (By Lot #) :


Research Areas :Cancer, Metabolic Disorder, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases, Cancer, Metabolic Disorder, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases