PRMT6, Active(P365-380FG)
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Description :Recombinant full-length human PRMT6 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_018137
Specific Activity :Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltransferase Assay
Molecular Weight :69 kDa
Gene Aliases :HRMT1L6
Scientific Background :PRMT6 (protein arginine methyltransferase 6), an arginine methyltransferase that exhibits type I PRMT activity and catalyzes the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. It can methylate itself. PRMT6 does not recognize the same substrates as PRMT4/CARM1 and displays limited substrate overlap with PRMT1. It resides in the nucleus along with PRMT1, PRMT2, and PRMT4, whereas PRMT3 and PRMT5 GFP fusions localize exclusively to the cytoplasm.
References :
1. Frankel A, et al: The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity. J Biol Chem. 277(5):3537-43, 2002.
2. Miranda TB, et al: Protein arginine methyltransferase 6 specifically methylates the nonhistone chromatin protein HMGA1a. Biochem Biophys Res Commun. 336(3):831-5, 2005.
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Research Areas :Apoptosis/Autophagy, Cancer, Inflammation, NfkB Pathway