Parkin Protein(P215-30G)
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Description :Recombinant full-length human Parkin was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :BC022014
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~68 kDa
Gene Aliases :PARK2, AR-JP, PDJ, PRKN, LPRS2
Scientific Background :Parkin or parkinson protein 2 is a RING domain-containing E3 ubiquitin protein ligase involved in proteasome-dependent degradation of proteins. Mutations in Parkin can lead to Parkinson’s disease. Parkin is important for mitochondrial quality control by lysosome-dependent degradation of damaged mitochondria through autophagy, or mitophagy (1). Parkin plays an important role in directly supporting mitochondrial function and protecting mitochondrial genomic integrity from oxidative stress (2). Parkin also has essential role in regulating normal respiratory function in mitochondria and in the protection of cells from oxidative stress.
References :
1. Yoshii, S. R. et.al: Parkin mediates proteasome-dependent protein degradation and rupture of the outer mitochondrial membrane. J. Biol. Chem. 286: 19630-19640, 2011.
2. Rothfuss, O. et.al: Parkin promotes mitochondrial genome integrity and supports mitochondrial DNA repair. Hum. Molec. Genet. 18: 3832-3850, 2009.
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Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Neurobiology, Cancer, Neurobiology, Apoptosis/Autophagy, Cell Cycle