NEK10, Active(N12-11G)
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Description : Recombinant human NEK10 (266-895) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :266-895
Genbank Number :NM_152534
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~98 kDa
Gene Aliases : NIMA (Never In Mitosis Gene A) Related Kinase 10
Scientific Background : NEK10 (NIMA Related Kinase 10) is a serine/threonine kinase that is involved in the regulation of the G2/M checkpoint in mitosis. It has unique structure with central catalytic kinase domain flanked by two large regulatory domains (1). NEK10 acts as a positive regulator of ERK1/2 in response to UV irradiation. In mitochondria, NEK10 interacts with GLUD1 and citrate synthase and plays a role in maintaining mitochondrial homeostasis (2). NEK10 also contributes to cilium formation through interaction with PKA and PCM1 and participating in a cAMP dependent pathway [3]. Mutations in NEK10 have been reported in lung cancer and breast cancer (2).
References :
1. Meirelles, G.V. et al: Stop Ne(c)king around: How interactomics contributes to functionally characterize Nek family kinases. World J Biol Chem. 5(2):141-60, 2014.
2. Peres de Oliveira, A. et al: NEK10 interactome and depletion reveal new roles in mitochondria. Proteome Sci. 18:4, 2020.
3. Porpora, M. et al: Counterregulation of cAMP-directed kinase activities controls ciliogenesis. Nat Commun. 9(1):1224, 2018.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cancer, Cell Cycle, Ser/Thr Kinases