MEKK4, Active (M12-11G)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description : Recombinant human MEKK4 (1203-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag : N Terminal GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1203-end
Genbank Number : NM_005922
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~72 kDa
Gene Aliases : MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412
Scientific Background : MEKK4 is one of the MAPKKKs which mediates activation of both p38 and JNK. N-terminal region of MEKK4 contains an autoinhibitory domain that interacts with the C-terminal kinase domain, inhibits kinase activity and prevents interaction with its substrate, MKK6. MEKK4 is activated upon binding of GADD45 by the stress-inducible GADD45 family of proteins (GADD45α/β/γ). GADD45 binds to the N-terminal region of MEKK4 releasing inhibition of the kinase domain by the autoinhibitory domain (1). MEKK4 is strongly expressed in the developing neuroepithelium and is critical for neural tube closure (2).
References :
1. Mita, H. et al: Regulation of MTK1/MEKK4 kinase activity by its N-terminal autoinhibitory domain and GADD45 binding. Mol Cell Biol. 2002; 22(13):4544-4555. doi:10.1128/mcb.22.13.4544-4555.2002.
2. Chi, H. et al: Loss of mitogen-activated protein kinase kinase kinase 4 (MEKK4) results in enhanced apoptosis and defective neural tube development. Proc Natl Acad Sci U S A. 2005; 102(10):3846-385.
Product Sheets (By Lot #) :
Research Areas :ERK/MAPK Pathway, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy, Invasion/Metastasis, Ser/Thr Kinases, ERK/MAPK Pathway, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy, Invasion/Metastasis, Ser/Thr Kinases