HERC4, Active(H265-381G)

HERC4, Active(H265-381G)

  • $215.00


Description :Recombinant human HERC4 (642-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :642-end

Genbank Number :NM_015601

Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Ubiquitin Enzyme Assay, Western Blot

Molecular Weight :71 kDa

Gene Aliases :KIAA1593

Scientific Background :HERC4 or HECT and RLD domain containing E3 ubiquitin protein ligase 4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD domain is predicted to act as a guanine nucleotide exchange factor for small G proteins. HERC4 is highly expressed in adult and fetal brain and in all specific brain regions examined.

References :
1. Hochrainer, K. et al: The human HERC family of ubiquitin ligases: novel members, genomic organization, expression profiling, and evolutionary aspects. Genomics 85: 153-164, 2005

2. Nagase, T. et al: Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 273-281, 2000

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Research Areas :AKT/PKB Pathway, Cancer, ERK/MAPK Pathway, Receptor Tyrosine Kinases