DTYMK, Active (D31-11G)
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Description: Recombinant human DTYMK (2-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species: Human
Tag : N-Terminal GST Tag
Expression System: Sf9 insect cells using baculovirus
Sequence: 2-end
Genbank Number :NM_012145
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Activity Assay, inhibition profile
Molecular Weight : 50 kDa
Gene Aliases : CDC8, PP3731, TMPK, TYMK, deoxythymidylate kinase
Scientific Background :DTYMK belongs to a family of thymidylate kinase proteins (1). DTYMK catalyzes phosphorylation of deoxy-TMP to the corresponding phosphate in the presence of ATP and magnesium. This enzyme is essential for cellular DNA synthesis and is an important intermediate enzyme in the pathway of many pyrimidine analog drugs (2). Elevated levels of dTMP have been correlated with poor survival in lung adenocarcinomas hence it is a potential anticancer therapeutic target (3).
References :
1. Su, J.Y. et al: Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast. Nucleic Acids Res. 1991; 19(4): 823–827.
2. Huang, S. et al: Human dTMP Kinase: Gene expression and enzymatic activity coinciding with cell cycle progression and cell growth. DNA and Cell Biology. 1994; 13(5):461-471.
3. Liu, Y. et al: Metabolic and functional genomic studies identify deoxythymidylate kinase as a target in LKB1-mutant lung cancer. Cancer Discov. 2013; 3(8):870-879.
Product Sheets (By Lot #) :
Research Areas :Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Inflammation, PKA/PKC Pathway