DGKH, Active(D26-10H)

DGKH, Active(D26-10H)

  • $226.00


Description : Recombinant full-length human DGKH was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Species :Human

Tag :His tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full length

Genbank Number : NM_178009.5.

Specific Activity : The specific activity of DGKH was determined to be 10 nmol/min/mg as per activity assay protocol.

Purity :The purity of DGKH was determined to be >85% by densitometry, calculated MW 139kDa.

Storage, Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles

Applications :Kinase Assay

Molecular Weight : 139 kDa

Gene Aliases : Diacylglycerol kinase eta; Diglyceride kinase eta; DGK-eta

Scientific Background :DGKH is a type II DGK encoded by gene DGKH (1). Diacylglycerol kinases modulate the concentration of intracellular diacylglycerol by phosphorylating, producing phosphatidic acid (PA) (2). PA regulates signaling proteins, such as protein kinases and phosphatases, and can induce cell mitosis through regulation of growth factors (3).  DGKH is a positive regulator of the epidermal growth factor receptor/Raf/MEK/ERK pathway. DGKH is associated with attention deficit hyperactivity disorder, unipolar depression and schizophrenia (4).

References :

  1. Rittiner, JE. et al: Overexpression of diacylglycerol kinase η enhances Gαq-coupled G protein-coupled receptor signaling. Mol Pharmacol. 2014; 85(5):800-810.
  2. Ding, L. et al: The cloning and characterization of a novel human diacylglycerol kinase, DGKiota. J Biol Chem. 1998; 273(49):32746-52.
  3. Buckland, AG. et al: Anionic phospholipids, interfacial binding and the regulation of cell functions. Biochim Biophys Acta. 2000; 1483(2):199-216.
  4. Sakane, F. et al: Diacylglycerol kinases as emerging potential drug targets for a variety of diseases: An update. Front Cell Dev Biol. 2016; 4:82

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Research Areas :Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Inflammation, PKA/PKC Pathway