DGKG, Active (D25-10G)
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Description :Recombinant full-length human DGKG was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_001346
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :116 kDa
Gene Aliases :DGKgamma, DAGK3, DAGKgamma
Scientific Background :Diacylglycerol kinase beta (DAG kinase beta) is a member of class I diacylglycerol kinase family and is regulated by Ca+ (1). Via converting diacylglycerol into phosphatidic acid, diacylglycerol kinases decrease the diacylglycerol levels to regulate the activity of PKC, Rho and Ras families (2). DGK beta is localized in brain and is involved in emotion and long-term memory linked to cognitive function (3). Impaired levels of diacylglycerol by DGK beta limits the NADPH oxidase activation in phagosomes (4).
References :
1. Sakane, F. et al. (1997) Diacylglycerol kinase J. Biol. Chem. 29, 1139-1143.
2. Kai, M.et al (2017). Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer. Mol. Carcinog. 56, 1743-1752.
3. Matsubara, T. et al. (2006). Nuclear transportation of diacylglycerol kinase γ and its possible function in the nucleus. J. Biol. Chem. 281, 6152-6164.
Product Sheets (By Lot #) :
Research Areas :Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Inflammation, PKA/PKC Pathway