DGKA, Active (D21-10CH)

  • $226.00


Description : Recombinant full-length rat DGKA was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Species : Rat

Tag : N-Terminal His Tag

Expression System:Sf9 insect cells using baculovirus

Sequence : Full length

Genbank Number :NM_080787

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, inhibition profile

Molecular Weight : 83 kDa

Gene Aliases : Dagk1

Scientific Background :Diacylglycerol kinase alpha (DAG kinase alpha) is a member of the eukaryotic diacylglycerol kinase family. Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resyn-thesis of phosphatidylinositols and attenuating protein kinase C activity. Involved in hepatocellular carcinoma progression through regulation of the Ras/Raf/MEK/ERK pathway. The DGKα/atypical PKC/β1 integrin signaling pathway is required for matrix invasion of breast carcinoma cells.

References :
1. Takeishi, K. et al. (2012). Diacylglycerol kinase alpha enhances hepatocellular carcinoma progression by activation of Ras-Raf-MEK-ERK pathway. J. Hepatol. 57, 77–83.
2. Rainero, E. et al. (2014). The diacylglycerol kinase alpha/atypical PKC/beta1 integrin pathway in SDF-1alpha mammary carcinoma invasiveness. PLoS ONE 9:e97144.
3. Sakane F, et al. (2016). Diacylglycerol Kinases as Emerging Potential Drug Targets for a Variety of Diseases: An Update. Front Cell Dev Biol. 4:82.

Product Sheets (By Lot #) :


Research Areas :Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Inflammation, PKA/PKC Pathway