CyclinM Protein(C89-30G)
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Description :Recombinant full-length human CyclinM was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_152274
Alternative Name(s) :CCNQ, CycM ; FAM58A
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :Cyclin M, the only conventional cyclin partner of CDK10, protects CDK10 from ubiquitin-mediated degradation (1). Cyclin M exhibits strong sequence homology to transcriptional cyclins, especially the L-type cyclins that are associated with CDK11, 12 and 13 to regulate transcription and splicing (2). CDK10 is required to form a complex with Cyclin M in order to phosphorylate Histone H1, the canonical CDK substrate, and ETS2, a transcription factor that plays key roles in cancer and development (2).
References :1. Khanal, P. et al: Proyl isomerase P1 facilitates ubiquitin-mediated degradation of cyclin-dependent kinase 10 to induce tamoxifen resistance in breast cancer cells. Oncogene. 2012, 31:3845-56. 2. Guen, V.J. et al. The awakening of the CDK10/Cyclin M protein kinase. Oncotarget. 2017, 8(30):50174-50186.
Purity :
Molecular Weight :Observed MW ~54 kDa. Calculated MW ~54 kDa.
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