CK1A1 (E42C), Active (C64-12CG)

CK1A1 (E42C), Active (C64-12CG)

  • $226.00


Description : Full-length recombinant human CK1a1 (E42C) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species : Human

Tag : GST tag

Expression System: Sf9 insect cells using baculovirus

Sequence : Full Length

Genbank Number : NM_001892

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications : Kinase Assay, Western Blot

Molecular Weight : ~62 kDa

Gene Aliases : CSNK1A1, CK1, HLCDGP1, PRO2975

 Scientific Background : CK1a1 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes. CK1 can regulate Smo cell surface accumulation and activity in response to hedgehog. CKI phosphorylate Smo at several sites and phosphorylation-deficient forms of Smo fail to accumulate on the cell surface and are unable to transduce the hedgehog signal (1). CK1a1 dynamically associates with the CBM complex on T cell receptor engagement to participate in cytokine production and lymphocyte proliferation. CK1a1 can form complex with MDM2 which then regulates the stability of p53 and E2F-1 transcription factors (2).

References :

  1. Jia, J. et al: Hedgehog signalling activity of Smoothened requires phosphorylation by protein kinase A and casein kinase I. Nature 432: 1045-1050, 2004. 
  2. Huart, A S. et al: CK1alpha plays a central role in mediating MDM2 control of p53 and E2F-1 protein stability. J Biol Chem. 2009 Nov 20;284(47):32384-94.

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