CDK10/CyclinM Protein(C41-34G)
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Description: Recombinant full-length human CDK10 and CyclinM proteins were co-expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Species : Human
Tag :GST tags
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_052988
Genbank Number 2 :NM_152274
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Enzyme Assay, Western Blot
Molecular Weight :CDK10 ~67 kDa, and Cyclin M ~52 kDa
Gene Aliases : CDK10: ALSAS, PISSLRE, CyclinM: CCNQ, CycM, FAM58A
Scientific Background : Cyclin-dependent kinase 10 (CDK10) belongs to cdc2 family of kinases and regulates the cell cycle (1). Cyclin M, the only conventional cyclin partner, protects CDK10 from ubiquitin mediated degradation (2). CDK10/cyclinM positively regulates the degradation of ETS2 through phosphorylation of two neighboring serines. EST2 is a transcription factor that plays key roles in cancer and development. CDK10/CyclinM is also known to repress ciliogenesis and to maintain actin cytoskeletan through the phoshorylation of the PKN2 protein kinase and the control of RhoA stability (3).
References :
1. Li, S. et. al: The cdc-2-related kinase, PISSLRE, is essential for cell growth and acts in G2 phase of the cell cycle. Cancer Res. 55:3992–3995, 1995.
2. Khanal, P. et. al: Proyl isomerase Pin1 facilitates ubiquitin-mediated degradation of cyclin-dependent kinase 10 to induce tamoxifen resistance in breast cancer cells. Oncogene. 31:3845–56, 2012.
3. Guen, V. J. et. al: The awakening of the CDK10/Cyclin M protein kinase. Oncotarget. 8(30), 50174–50186, 2017.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases