CDK9/CyclinT1, Active(C40-18CG)

CDK9/CyclinT1, Active(C40-18CG)

  • $226.00


Description :Recombinant full-length human CDK9 and CyclinT1 proteins were co-expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System: Sf9 insect cells using baculovirus

Sequence :Full length

Genbank Number :NM_001261

Genbank Number 2 :NM_001240

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~68 kDa/110 kDa

Gene Aliases :CDK9: CDC2L4, C-2k, TAK, PITALRE . CyclinT1:  CCNT; CYCT1; HIVE1

Scientific Background :Cyclin-dependent kinase 9 (Cdk9) is a cdc2-like serine/threonine kinase that associates with cyclin T1 to form a heterodimer. CDK9/Cyclin T1 heterodimer is the main component of the positive transcription elongation factor (P-TEFb) which is critical for the stabilization of the elongation process of RNA polymerase II generated transcripts (1). P-TEFb containing Cdk9 and cyclin T1 is essential for the replication of the human immunodeficiency virus type 1 and type 2 in human cells (2). Cdk9- associated pathway are linked to human malignancies and cardiac hypertrophy (3).

References :

1. Gaetano, R. et. al: Role of the cyclin-dependent kinase 9-related pathway in mammalian gene expression and human diseases. Cell Cycle. 7:23, 3664-3668, 2008.
2. Lin, X. et. al: P-TEFb containing cyclin K and Cdk9 can activate transcription via RNA. J Biol Chem. 277:16873-8, 2002.
3. Malumbres, M. et. al: CDK inhibitors in cancer therapy: what is next? Trends Pharmacol Sci. 29:16-21, 2008.

Product Sheets (By Lot #) :


Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cancer, Cell Cycle, Ser/Thr Kinases