CDK9/CyclinT1, Active(C40-18CG)
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Description :Recombinant full-length human CDK9 and CyclinT1 proteins were co-expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System: Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_001261
Genbank Number 2 :NM_001240
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~68 kDa/110 kDa
Gene Aliases :CDK9: CDC2L4, C-2k, TAK, PITALRE . CyclinT1: CCNT; CYCT1; HIVE1
Scientific Background :Cyclin-dependent kinase 9 (Cdk9) is a cdc2-like serine/threonine kinase that associates with cyclin T1 to form a heterodimer. CDK9/Cyclin T1 heterodimer is the main component of the positive transcription elongation factor (P-TEFb) which is critical for the stabilization of the elongation process of RNA polymerase II generated transcripts (1). P-TEFb containing Cdk9 and cyclin T1 is essential for the replication of the human immunodeficiency virus type 1 and type 2 in human cells (2). Cdk9- associated pathway are linked to human malignancies and cardiac hypertrophy (3).
References :
1. Gaetano, R. et. al: Role of the cyclin-dependent kinase 9-related pathway in mammalian gene expression and human diseases. Cell Cycle. 7:23, 3664-3668, 2008.
2. Lin, X. et. al: P-TEFb containing cyclin K and Cdk9 can activate transcription via RNA. J Biol Chem. 277:16873-8, 2002.
3. Malumbres, M. et. al: CDK inhibitors in cancer therapy: what is next? Trends Pharmacol Sci. 29:16-21, 2008.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cancer, Cell Cycle, Ser/Thr Kinases