BRSK2, Active(B14-10G)
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Description :Recombinant full-length human BRSK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_003957
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~108 kDa
Gene Aliases :HUSSY-12, C11orf7; PEN11B; SAD1; STK29
Scientific Background :BRSK2 is a brain-selective serine/threonine kinase 2 that is mainly expressed in the brain, with weaker expression in testis and pancreas. BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C and CDC25B in an in vitro assay. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhanced endogenous BRSK2 kinase activity in a caffeine-sensitive manner and caused translocation of BRSK2 from the cytoplasm to the nucleus (1). Overexpression of BRSK2 induces G2/M arrest in HeLa cells while small interfering RNA against BRSK2 partly abrogated UV-induced G2/M arrest. Mammalian BRSK2 kinases are required for neuronal polarization (2).
References :
1. Lu, R. et.al: Human SAD1 kinase is involved in UV-induced DNA damage checkpoint function. J. Biol. Chem. 279: 31164-31170, 2004.
2. Kishi,M.et.al: Mammalian SAD kinases are required for neuronal polarization. Science 307: 929-932, 2005.
Product Sheets (By Lot #) :
Research Areas :Ser/Thr Kinases, Metabolic Disorder, Cellular Stress, Cancer, Apoptosis/Autophagy, Cancer, Metabolic Disorder, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases