BRAF (d486-490 V471F) Protein (B08-12LG)

BRAF (d486-490 V471F) Protein (B08-12LG)

  • $226.00


Description :Recombinant human BRAF (d486-490 V471F) (381-end or exon 9-18) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :381-end or exon 9-18 (d486-490 V471F)

Genbank Number :NM_004333

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay

Molecular Weight :~69 kDa

Gene Aliases :BRAF (LP485_490Y), BRAF (Ex9-18), BRAF1, RAFB1, B-raf, B-raf 1, MGC126806, MGC138284

Scientific Background :BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRAF in cells responding to growth factor signals (1). Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of BRAF (2).

References :
1. Weber, C K. et al: Active Ras induces heterodimerization of cRaf and BRaf. Cancer Res. 2001 May 1;61(9):3595-8.

2. Mercer, K E. et al: Raf proteins and cancer: B-Raf is identified as a mutational target. Biochim Biophys Acta. 2003 Jun 5;1653(1):25-40.

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Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, JAK/STAT Pathway, Ser/Thr Kinases