MEK3 (MAP2K3), Unactive(M04-14G)
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Description :Recombinant full-length human MEK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_002756
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :62 kDa
Gene Aliases :MAP2K3, MEK3, MAPKK3, PRKMK3, SKK2
Scientific Background :Mitogen-acitvated protein kinase kinase 3 (MEK3) is a serine/threonine kinase that is involved in the activation of MAP kinases. MEK3 is a dual-specificity protein kinase that phosphorylates p38 MAP kinase at its activation site Thr-Gly-Tyr and this stimulates the ability of p38 MAPK to phosphorylate protein substrates such as ATF-2 and Elk-1. MEK3 is activated by different forms of cellular stress and inflammatory cytokines. MEK3-null mice are viable and fertile, but their macrophages and dendritic cells are defective in IL-12 production (1). A virulence factor from Yersinia pseudotuberculosis, YopJ, was found to bind directly to MEK3 in vitro and this leads to blockage of its phosphorylation and activation (2).
References :
1. Lu, H.-T. et al: Defective IL-12 production in mitogen-activated protein (MAP) kinase kinase 3 (MEK3)-deficient mice. EMBO J. 18: 1845-1857, 1999.
2. Orth, K. et al: Inhibition of the mitogen-activated protein kinase kinase superfamily by a Yersinia effector. Science 285: 1920-1923, 1999.
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Research Areas :Cancer, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Invasion/Metastasis, Ser/Thr Kinases, Angiogenesis