UBE2F Protein(U220-30H)
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Description :Recombinant full length human UBE2F was expressed in E. coli cells using an N-terminal His tag.
Species :Human
Tag :HIS tag
Expression System:E.coli
Sequence :Full Length
Genbank Number :NM_080678
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~24 kDa
Gene Aliases :NCE2
Scientific Background :UBE2F or ubiquitin-conjugating enzyme E2F is part of the ubiquitin network for protein degradation and is involved in protein neddylation. UBE2F has been shown to function as a NEDD8-conjugating enzyme in vitro and in vivo (1). Covalent attachment of the ubiquitin-like protein NEDD8 to a conserved C-terminal domain (ctd) lysine stimulates Cullin Ring ligase (CRL) ubiquitination activity and prevents binding of the inhibitor CAND1 (2).
References :1. Huang, D T. et al: E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol Cell. 2009 Feb 27;33(4):483-105. 2. Boh, B K. et al: Neddylation-induced conformational control regulates cullin RING ligase activity in vivo. J Mol Biol. 2011 Jun 3;409(2):136-45.
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Research Areas :Neurobiology, Cell Cycle, Cancer