UBE2D2 (UBC4) Protein(U214-30H)
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Description :Recombinant full length Xenopus UBE2D2 (UBC4) was expressed in E. coli cells using an N-terminal His tag.
Species :Xenopus
Tag :HIS tag
Expression System:E.coli
Sequence :Full Length
Genbank Number :NM_001093036
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~17 kDa
Gene Aliases :UBC4; PUBC1; UBC4/5; UBCH5B; E2(17)KB2
Scientific Background :UBE2D2(UBC4) Protein or ubiquitin-conjugating enzyme E2D 2 is a member of the E2 ubiquitin-conjugating enzyme family which functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 specifically ubiquitinates E6AP and in vivo inhibition of UBE2D2 can lead to inhibition of E6-stimulated p53 degradation (1). UBE2D2 could conjugate ubiquitin to target proteins in an E6AP-dependent manner (2).
References :
1. Rolfe, M.et.al: Reconstitution of p53-ubiquitylation reactions from purified components: the role of human ubiquitin-conjugating enzyme UBC4 and E6-associated protein (E6AP). Proc. Nat. Acad. Sci. 92: 3264-3268, 19105.
2. Jensen, J. P.et.al: Identification of a family of closely related human ubiquitin conjugating enzymes. J. Biol. Chem. 270: 30408-30414, 19105.
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Research Areas :Neurobiology, Cell Cycle, Cancer