RanGAP1 Protein(R298-31H)
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Description :Recombinant human RanGAP1 (418-end) was expressed in E. coli cells using an N-terminal his tag.
Species :Human
Tag :His tag
Expression System:E.coli
Sequence :418-end
Genbank Number :NM_002883
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~20 kDa
Gene Aliases :Fug1; RANGAP; SD
Scientific Background :RANGAP1, Ran GTPase activating protein 1, is the control substrate, which produces a single SUMO modification during SUMOylation (1). As a GTPase activator, RANGAP1 is involved in nuclear envelope assembly and nuclear transport through interacting with Ras-related nuclear protein 1(RAN) and regulating GTP-binding and exchange (2). Also, abnormal localization of RanGAP1 was found in cortex of the patients with Huntington’s disease (3)
References :
1. Joseph J., et.al. SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles. J. Cell Biol. 156:595-602, 2002.
2. Swaminathan S., et.al. RanGAP1*SUMO1 is phosphorylated at the onset of mitosis and remains associated with RanBP2 upon NPC disassembly. J. Cell Biol. 164:965-971, 2004.
3. Gasset-Rosa F., et.al. Polyglutamine-Expanded Huntingtin Exacerbates Age-Related Disruption of Nuclear Integrity and Nucleocytoplasmic Transport. Neuron. Apr 5; 94 (1):48-57, 2017.
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Research Areas :Cancer, Cell Cycle, Neurobiology