NAE1 Complex, Protein (N209-31H)
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Description : Recombinant human NAE1 (2-end) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Species :Human
Tag : HIS/GST tag
Expression System: Sf9 insect cells using baculovirus
Sequence : Full length
Genbank Number : NM_003905
Purity : The purity of NAE1 was determined to be >70% by densitometry. Approx. MW 65 kDa.
Storage, Stability, and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Ubiquitin Enzyme Assay, Western Blot
Molecular Weight : 65kDa approx.
Gene Aliases : APPBP1
Scientific Background : NAE1 is the regulatory subunit of heterodimeric NAE1/UA3 E1 (Ub-activating)–like complex. In the presence of ATP, NAE1/UBA3 adenylates and activates NEDD8 yielding NEDD8-UBA3 thioester and free AMP. Activated NEDD8 is then transferred to UBE2M or UBE2F through transthiolyation reaction. Finally, substrate specific-E3 ligase, such as RBX1/2 or DCN1, transfers NEDD8 from UBE2M or UBE2F to a lysine residue in its target protein (1,2). NEDD8 pathway plays an important role in cell cycle progression and survival (2).
References :
- Zhou, L. et al: Neddylation: a novel modulator of the tumor microenvironment. Mol Cancer. 18: 77, 2019.
- Gong, L. et al: Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. J Biol Chem. 23 (274):12036-42, 1999.
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Research Areas :Cancer, Cell Cycle, Neurobiology