HERC4, Active(H265-381G)
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Description :Recombinant human HERC4 (642-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :642-end
Genbank Number :NM_015601
Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Ubiquitin Enzyme Assay, Western Blot
Molecular Weight :71 kDa
Gene Aliases :KIAA1593
Scientific Background :HERC4 or HECT and RLD domain containing E3 ubiquitin protein ligase 4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD domain is predicted to act as a guanine nucleotide exchange factor for small G proteins. HERC4 is highly expressed in adult and fetal brain and in all specific brain regions examined.
References :
1. Hochrainer, K. et al: The human HERC family of ubiquitin ligases: novel members, genomic organization, expression profiling, and evolutionary aspects. Genomics 85: 153-164, 2005
2. Nagase, T. et al: Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 273-281, 2000
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Research Areas :AKT/PKB Pathway, Cancer, ERK/MAPK Pathway, Receptor Tyrosine Kinases