LC20 Protein(M89-54G)
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Description :Recombinant full-length mouse LC20 was expressed in E. coli cells using an N-terminal GST tag.
Species :Mouse
Tag :GST tag
Expression System:E.coli
Sequence :Full Length
Genbank Number :BC055439
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~45 kDa
Gene Aliases :MYL9, MGC3505, MLC2, MRLC1, MYRL2
Scientific Background :LC20 is the myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads (1). LC20 protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for LC20 and the deduced 172-amino acid protein is highly conserved, with only 3 differences between the human and chicken proteins. Light chain phosphorylation causes the folded monomeric form of myosin to extend and assemble into filaments. This observation established the involvement of the LC20 in conformational transitions of smooth muscle myosin (2).
References :
1. Wagner, P D. et al: Regulation of the actin-activated ATPase of aorta smooth muscle myosin. J Biol Chem. 1986 Jun 15;261(17):7778-83.
2. Trybus, K M. et al: The regulatory light chain is required for folding of smooth muscle myosin. J Biol Chem. 1988 Nov 5;263(31):16485-92.
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Research Areas :Cardiovascular Disease, Cardiovascular Disease