TDP43 Protein(T74-34G)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Recombinant full-length human TDP43 protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_007375
Alternative Name(s) :ALS10; TARDBP
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :TAR DNA binding protein (TARDBP) Human was originally discovered in a screen for transcriptional repressors of the transactive response (TAR) DNA binding element of the HIV-1 virus. Contain two RNA-recognition motifs (RRM1 and RRM2), one of which partially overlaps with a putative nuclear export sequence. Mutations in TARDBP were identified in amyotrophic lateral sclerosis (ALS) patients.
References :1. Ou SH, et al: Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs. J Virol 69:3584–3596. 1995
2. Gitcho MA, et al. TDP-43 A315T mutation in familial motor neuron disease. Ann Neurol 2008 63:535–538. 2008
Purity :
Molecular Weight :Observed MW ~73 kDa. Calculated MW ~73 kDa.
Product Sheets (By Lot #) :