Tau-441 (P301S) Pre-Formed Fibril(T08-58GN)
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Description :Recombinant full-length tag-free human Tau-441(P301S) Pre-Formed Fibril was expressed in E. coli cells.
Species :Human
Tag :TAG-Free
Expression System:E.coli
Sequence :Full Length
Genbank Number :NP_005901.2
Alternative Names :Microtubule-associated protein tau; MAPT; MAP; Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1; Isoform 2;
Formulation :Recombinant protein stored in 10mM HEPES, pH 7.4, 100mM NaCl.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). 2N4R, or Tau-441 is the full length tau protein. P301S is a mutation encoded by exon 10 (4) that impairs the ability of tau to assemble microtubules (5).
References :1. www.alz.org/alzheimers-dementia/facts-figures
2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907)
3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497.
4. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18.
5. Bugiani, O. et al. (1999). J Neuropathol Exp Neurol. 58(6):667-77.
Sample Data :SDS-PAGE of ~67 kDa Human Tau-441 (P301S) Pre-Formed Fibrils (Cat# T08-58GN). Lane 1: MW Ladder. Lane 2: Tau-441 Pre-Formed Fibrils (Cat# T08-58GN).
Sample Data :TEM of recombinant Tau-441 (P301S) Pre-Formed Fibrils (Cat# T08-58GN) at 150kx magnification. HV = 80kV. Fibrils were sonicated and stained with uranyl acetate.
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