SMAD4 Protein(S13-30G)
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Description :Recombinant full-length human SMAD4 was expressed in E. coli cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:E.coli
Sequence :Full Length
Genbank Number :NM_005359
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.gel.gif)
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~95 kDa
Gene Aliases :JIP, DPC4, MADH4
Scientific Background :SMAD4 is a member of the SMAD family and mediates signaling by the transforming growth factor-beta (TGFβ) superfamily and related ligands (1). TGFβ stimulation leads to phosphorylation and activation of SMAD1, SMAD2 and SMAD3, which form complexes with SMAD4 that accumulate in the nucleus and regulate transcription of target genes. SMAD signaling is negatively regulated by inhibitory SMADs and ubiquitin-mediated processes and proteasomal degradation of SMADs depend on the direct interaction of specific E3 ligases with SMADs. SMAD4 is targeted for degradation by multiple ubiquitin ligases that can simultaneously act on R-SMADs and signaling receptors. Such mechanisms of down-regulation of TGFβ signaling via degradation of SMADs may be critical for proper physiological response to this pathway (2).
References :
1. Heldin, C.H. et al: TGF-beta signalling from cell membrane to nucleus through SMAD proteins". Nature, 1997, 390 (6659): 465–71.
2. Attisano, L. et al: Mads and Smads in TGF beta signalling". Curr. Opin. Cell Biol. 1998; 10 (2): 188–94.
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Research Areas :Cancer, Inflammation, AKT/PKB Pathway, ERK/MAPK Pathway, JNK/SAPK Pathway, NfkB Pathway, JAK/STAT Pathway, WNT Signaling, Cell Cycle, Cellular Stress, Angiogenesis, Cancer, Inflammation, AKT/PKB Pathway, ERK/MAPK Pathway, JNK/SAPK Pathway, NfkB Pathway, JAK/STAT Pathway, WNT Signaling, Cell Cycle, Cellular Stress, Angiogenesis