RIPK1, Active(R07D-112G)
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Description :Recombinant dog RIPK1 (1-373) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Dog
Tag :GST
Expression System:Sf9 insect cells using baculovirus
Sequence :1-373
Genbank Number :A0A8I3S2L7
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, and 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :RIPK1 or Receptor Interacting Protein Kinase 1 is a serine/threonine kinase that was originally identified as interacting with the cytoplasmic domain of FAS. RIPK1 has been deemed as an important element in the signal transduction machinery that mediates programmed cell death. RIPK1 has been shown to interact with TRADD, TRAF1 TRAF2 and TRAF3 and TRADD can act as an adaptor protein to recruit RIPK1 to the TNFR1 complex in a TNF-dependent process (1). TNFα is capable of activating the noncanonical NF-κB pathway, but this activation of this pathway is negatively regulated by RIPK1 (2).
References :1. Hsu, H. et al: TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex. Immunity 4: 387-396, 1996.
2. Kim, J Y. et al: TNFα induced noncanonical NF-κB activation is attenuated by RIP1 through stabilization of TRAF2. J Cell Sci. 2011 Feb 15;124 (Pt 4):647-56.
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Observed MW ~69 kDa.
Calculated MW ~68 kDa.
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