KRAS (G12D, Isoform B) Protein(R06B-32BH)
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Description :Recombinant human KRAS (G12D, Isoform B) (1-186) was expressed in E. coli cells using an N-terminal His tag. KRASB (G12D) is constitutively active.
Species :Human
Tag :HIS
Expression System:E.coli
Sequence :(1-186)
Genbank Number :NM_004985
Alternative Name(s) :C-K-RAS; c-Ki-ras; c-ki-ras2; CFC2; K-Ras; K-Ras 2; K-RAS2B; K-RAS4B; KI-RAS; KRAS; KRAS2; NS; NS3; OES; RALD; RASK2
Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, and 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking (1). There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types (2). Other members of the RAS superfamily may also contribute to cancer. Various studies indicate that expression of endogenous KRAS isoform B plays a major role in development (3, 4).
References :1. Shih, T. Y., et al: Structure and function of p21 ras proteins. Gene Amplif Anal. 1986;4:53-72.
2. Rodriguez-Viciana, P.: Cancer targets in the Ras pathway. Cold Spring Harb Symp Quant Biol. 2005;70:461-7.
3. Carta, C. et al: Germline Missense Mutations Affecting KRAS Isoform B Are Associated with Severe Noonan Syndrome Phenotype. Am J Hum Genet. 2006;79(1):129-135.
4. Salmon, M. et al: KRAS4A induces metastatic lung adenocarcinomas in vivo in the absence of the KRAS4B isoform. Proc Natl Acad Sci USA. 2021;118(30): 10.1073/pnas.2023112118.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Calculated MW ~21 kDa.
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Research Areas :Cell Cycle, Cellular Stress