Rb (N-Term) Protein(R05-56G)
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Description :Recombinant human Rb (48-378) was expressed in E. coli cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:E.coli
Sequence :48-378
Genbank Number :NM_000321
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~62 kDa
Gene Aliases :RB1, OSRC
Scientific Background :Rb is the retinoblastoma gene product and was the first tumor suppressor cloned. Rb is a negative regulator of the cell cycle through its ability to bind the transcription factor E2F and repress transcription of genes required for S phase (1). Rb can bind to MDM2 and this overcomes both the antiapoptotic function of MDM2 and the MDM2-dependent degradation of p53. Rb associates with the Polycomb group (PcG) proteins to form a repressor complex that blocks entry of cells into mitosis (2). Rb colocalizes with nuclear PcG complexes and is important for association of PcG complexes with nuclear targets.
References :
1. Hanahan, D.et al: The hallmarks of cancer. Cell 100: 57-70, 2000.
2. Dahiya, A. et al: Linking the Rb and Polycomb pathways. Molec. Cell 8: 557-568, 2001.
Product Sheets (By Lot #) :
Research Areas :Cancer, Neurobiology, Cell Cycle, Cancer, Neurobiology, Cell Cycle