PRMT5/MEP50, Active(P329-310EG)
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Description :Recombinant full-length human PRMT5 and MEP50 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Species :Human
Tag :GST
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_006109
Genbank Number 2 :BC001679
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Alternative Name(s) :PRMT5: HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs MEP50: WDR77; WD45
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :PRMT5 (Protein Arginine Methyltransferase 5) and its coactivator MEP50 (Methylosome Protein 50) form a complex that plays a crucial role in the methylation of arginine residues in proteins, a process known as arginine methylation. This complex has been implicated in the regulation of various cellular functions, including gene expression, RNA processing, and signal transduction. Aberrant expression and dysregulation of PRMT5 and MEP50 have been observed in various cancers, highlighting their potential roles in tumorigenesis.
References :1. Yang Y, Bedford MT. (2013) Protein arginine methyltransferases and cancer. Nat Rev Cancer. 13(1):37-50.
2. Blanc RS, Richard S. (2017) Arginine Methylation: The Coming of Age. Mol Cell. 65(1):8-24.
3. Stopa N, Krebs JE, Shechter D. (2015) The PRMT5 arginine methyltransferase: many roles in development, cancer and beyond. Cell Mol Life Sci. 72(11):2041-2059.
Molecular Weight :PRMT5 observed MW ~100 kDa. PRMT5 calculated MW ~99 kDa.
MEP50 observed MW ~65 kDa. MEP50 calculated MW ~65 kDa.
Product Sheets (By Lot #) :