PCNA Protein(P11-30H)
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Description :Recombinant full-length human PCNA was expressed by E. coli cells using an N-terminal His tag.
Species :Human
Tag :HIS tag
Expression System:E.coli
Sequence :Full Length
Genbank Number :NM_002592
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.gel.gif)
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~37 kDa
Gene Aliases :MGC8367
Scientific Background :PCNA is a nuclear protein whose appearance correlates with the proliferative state of the cells and is a cofactor of DNA polymerase delta. PCNA is a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Immunofluorescence studies have shown that p300 may play a role in DNA repair synthesis through its interaction with PCNA. In vitro and in vivo p300 forms a complex with PCNA that does not depend on the S phase of the cell cycle and stimulates DNA synthesis in vitro (1). PCNA interacts with the Williams syndrome transcription factor (WSTF) allowing it to target to DNA replication foci, that then allows recruitment of SNF2H (2).
References :
1. Hasan, S. et al: Transcription coactivator p300 binds PCNA and may have a role in DNA repair synthesis. Nature 410: 387-391, 2001.
2. Poot, R. A.et al: The Williams syndrome transcription factor interacts with PCNA to target chromatin remodelling by ISWI to replication foci. Nature Cell Biol. 6: 1236-1244, 2004.
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Research Areas :WNT Signaling, ERK/MAPK Pathway, Cell Cycle, Cancer, Cancer, ERK/MAPK Pathway, WNT Signaling, Cell Cycle