NCK Protein (N06-30H)
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Description : Recombinant full-length human NCK was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Species : Human
Tag : HIS tag
Expression System : Sf9 insect cells using baculovirus
Sequence : Full length
Genbank Number : NM_006153
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Western Blot
Molecular Weight : ~48 kDa
Gene Aliases : NCK1, MGC12668, NCKalpha
Scientific Background : NCK (or non-catalytic region of tyrosine kinase adaptor protein 1) is an adaptor protein that is located in the cytoplasm and involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS (1). NCK is a signaling and transforming protein that contains Src homology 2 and 3 (SH2 and SH3) domains. NCK functions by coupling tyrosine phosphorylation via SH2 domains to downstream effectors through SH3 domains. Furthermore, NCK couples tyrosine phosphorylation guidance signals to cytoskeletal events required for the ipsilateral projections of spinal cord neurons and for normal limb movement (2).
References :
1. Park, D. et al: Phosphorylation of Nck in response to a variety of receptors, phorbol myristate acetate, and cyclic AMP. Mol. Cell. Biol.1992; 12 (12): 5816–23.
2. Fawcett, J. P. et al: Nck adaptor proteins control the organization of neuronal circuits important for walking. Proc. Nat. Acad. Sci. 104: 20973-20978, 2007
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Research Areas : Angiogenesis, Cancer, Inflammation, Invasion/Metastasis,