MYCN Protein(M86-34G)
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Description :Recombinant full-length human MYCN was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NP_005369.2
Alternative Name(s) :bHLHe37; MODED; MYCNsORF; MYCNsPEP; N-myc; NMYC; ODED
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :MYCN is a basic helix-loop-helix transcription factor first identified in neuroblastoma and is a member of the MYC proto-oncogene family along with MYCC and MYCL. MYC proteins affect transcription of many genes thus regulating proliferation, metabolism, apoptosis, differentiation and immune surveillance. Ubiquitin-dependent proteasome degradation of MYCN in neural stem/progenitor cells is required for the arrest of proliferation and the start of differentiation. Enhanced USP7 expression is seen in neuroblastoma patients with poorer prognosis due to deubiquination of MYCN by USP7, thereby preventing degradation of MYCN (1). Overexpression of MYCN is closely related with high-cancers, early distant metastasis, and poor clinical prognosis (2).
References :1. Liu, R. et al: Molecular Mechanisms of MYCN Dysregulation in Cancers. Front Oncol. 2020, 10:625332.
2. Yang, Y. et al: MYCN protein stability is a better prognostic indicator in neuroblastoma. BMC Pediatr. 2022, 22:404.
Purity :
Molecular Weight :Observed MW ~95 kDa. Calculated MW ~80 kDa.
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