MYT1, Active(M67-10H)
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Description :Recombinant full-length human MYT1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Species :Human
Tag :HIS
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_004203
Alternative Name(s) :PKMYT1, PPP1R126
Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :MYT1 is a member of a family of neural specific, zinc finger-containing DNA-binding proteins which binds to the promoter regions of proteolipid proteins of the central nervous system and plays a role in the developing nervous system. MYT1 of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system (1). The recombinant MYT1 fragments containing either the upstream 2 zinc fingers or the downstream 4 zinc fingers bound the same cis regulatory element in the PLP1 promoter in vitro and DNA binding required Zn (2+), but not other divalent cations (2).
References :1. Kim, J. G. et.al: Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. J. Neurosci. Res. 50: 272-290, 1997.
2. Kim, J. G. et.al: Novel member of the zinc finger superfamily: a C (2)-HC finger that recognizes a glia-specific gene. Molec. Cell. Biol. 12: 5632-5639, 1992.
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Observed MW ~65 kDa.
Calculated MW ~58.7 kDa.
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