MET, Active(M52-18H)
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Description :Recombinant human MET (956-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Species :Human
Tag :HIS
Expression System:Sf9 insect cells using baculovirus
Sequence :956-end
Genbank Number :NM_000245
Alternative Name(s) :AUTS9, c-MET, DA11, DFNB97, HGFR, RCCP2
Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :MET is a proto-oncogene that encodes a transmembrane growth factor receptor which is a heterodimer of two disulphide linked chains of 50 kDa (alpha) and 145 kDa (beta). MET is widely expressed in the kidney, brain, lung, skin, and embryonic tissue (1). Hepatocyte growth factor (HGF) binds to MET and activates its tyrosine kinase activity. MET is overexpressed and activated in a variety of human cancers including pancreatic, colon, gastric, cervical and ovarian cancers and has been shown to be involved in tumor cell migration and invasion (2).
References :1. Giordano, S. et al: Biosynthesis of the protein encoded by the c-met proto-oncogene. Oncogene. 1989 Nov;4(11):1383-8.
2. Iyer, A. et al: Structure, tissue-specific expression, and transforming activity of the mouse met protooncogene. Cell Growth Differ. 1990 Feb;1(2):87-95.
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Observed MW ~60 kDa.
Calculated MW ~50 kDa.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Cellular Stress