IkBA Protein(I20-31G)
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Description :Recombinant human IkBA (1-175) was expressed in E. coli cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:E.coli
Sequence :1-175
Genbank Number :NM_020529
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~46 kDa
Gene Aliases :NFKBIA, MAD-3, NFKBI
Scientific Background :IκBα is an inhibitor of the NFκB complex and inactivates the NFκB by trapping it in the cytoplasm (1). Phosphorylation of serine residues on the IκB protein by IκB kinases (IKKs) marks it for destruction via the ubiquitination pathway, thereby allowing the activation of the NFκB complex. Synthetic glucocorticoid such as dexamethasone display anti-inflammatory effects by inducing the increased synthesis of the IκB protein thereby inhibiting the activity of the NFκB complex. Overexpression of the IκBα gene in fibroblasts leads to inhibition of production of IL-6, TNF receptor, MMP-1, MMP-3 and MMP-13 (2).
References :
1. Auphan, N. Et al: Immunosuppression by glucocorticoids: inhibition of NF-kappa-B activity through induction of I-kappa-B synthesis. Science 270: 286-290, 1995.
2. Bondeson, J. Et al: Adenoviral gene transfer of the endogenous inhibitor IkappaBalpha into human osteoarthritis synovial fibroblasts demonstrates that several matrix metalloproteinases and aggrecanases are nuclear factor-kappaB-dependent. J Rheumatol. 2007 Mar;34(3):523-33.
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Research Areas :Inflammation, AKT/PKB Pathway, p38 Pathway, Apoptosis/Autophagy, Inflammation, AKT/PKB Pathway, p38 Pathway, Apoptosis/Autophagy