FADD Protein (F02-30H)
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Description : Recombinant full-length human FADD was expressed in E. coli cells using an N-terminal His tag.
Species : Human
Tag : HIS tag
Expression System : E.coli
Sequence : Full length
Genbank Number : NM_003824
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Western Blot
Molecular Weight : ~27 kDa
Gene Aliases : GIG3, MORT1, MGC8528
Scientific Background : FADD or Fas-Associated protein with Death Domain is an adaptor molecule that mediates death signaling by the Fas-receptor, tumor necrosis factor receptor and TRAIL-receptor. FADD binds to these receptors via the C-terminus Death Domain which then unmasks the N-terminal effector domain of FADD thereby allowing it to recruit caspase-8 and activate the cysteine protease cascade leading to apoptosis (1). Cells lacking FADD are defective in intracellular double-stranded RNA (dsRNA)-activated gene expression, including production of type I (alpha/beta) interferons and are thus very susceptible to viral infection (2).
References :
1. Sheikh, M.S et al: The FADD is going nuclear. Cell Cycle, 2004; 2 (4): 346–7.
2. Balachandran, S. et al : A FADD-dependent innate immune mechanism in mammalian cells. Nature 432: 401-405, 2004.
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Research Areas : Apoptosis/Autophagy, Cancer, Cardiovascular Disease, NfkB Pathway,