HER3 Protein(E28-35G)
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Description :Recombinant human HER3 (684-1020) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST
Expression System:Sf9 insect cells using baculovirus
Sequence :684-1020
Genbank Number :P21860
Alternative Name(s) :erbB-3
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :HER3 is a member of the human epidermal growth factor receptor (EGFR/HER) tyrosine kinase family whose kinase domain lacks several residues critical for catalysis but serves as an activator of the EGFR kinase domain (1,2). Phosphorylation of HER3 by other EGFR family members, most commonly HER2, activates the PI3K/AKT pathway (2). In cancer cells treated with HER2 kinase inhibitors, diminished HER2 activity is compensated for by HER3 overexpression, resulting in tumor cell survival (2).
References :1. Shi, F. et al: ErbB3/HER3 intracellular domain is competent to bind ATP and catalyze autophosphorylation. Proc Natl Acad Sci USA 107(17): 7692-7697, 2010.
2. Jura, N. et al: Structural analysis of the catalytically inactive kinase domain of the human EGF receptor 3. Proc Natl Acad Sci USA 106(51): 21608-21613, 2009.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Calculated MW 64.2 kDa.
Product Sheets (By Lot #) :
Research Areas :Cancer, Receptor Tyrosine Kinases