DNAPK Protein(D08-35G)
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Description :Recombinant human DNAPK (3746-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :3746-end
Genbank Number :NM_006904
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :68 kDa
Gene Aliases :PRKDC, HYRC, p350, DNA-PK, DNPK1, HYRC1, XRCC7
Scientific Background :DNAPK is a member of the PI3/PI4-kinase family which encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK), which is involved in DNA nonhomologous end-joining (NHEJ) during DNA double-strand break (DSB) repair and for V(D)J recombination during immune development. The second component of DNA-PK is Ku, which is required for proper activation of PRKDC (1). The initial events in retroviral integration are detected as DNA damage by the host cell, and that completion of the integration process requires the DNA-PK-mediated repair pathway (2).
References :
1. Van der Burg, M.et.al: A DNA-PKcs mutation in a radiosensitive T-B- SCID patient inhibits Artemis activation and nonhomologous end-joining. J. Clin. Invest. 119: 91-98, 2009.
2. Daniel, R.et.al: A role for DNA-PK in retroviral DNA integration. Science 284: 644-647, 1999.
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Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cancer, Cell Cycle, Ser/Thr Kinases