CyclinD2 Protein(C81-30B2G)
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Description :Recombinant full-length human CyclinD2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_001759.4
Alternative Name(s) :CCND2; MPPH3; KIAK0002
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT and 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :CyclinD2 is a member of the D-type cyclins. D-type cyclins are regulators of the cell cycle and interact with several transcription factors modulating their transcriptional activations. CyclinD2 forms a complex and functions as a regulatory subunit of CDK4 or CDK6 that regulates the cell-cycle during G1/S transition. These complexes overcome the G1 growth arrest phase by phosphorylating and inactivating the retinoblastoma tumor suppressor protein (1). CyclinD2 is upregulated in the carcinogenesis of tumors, making it a possible therapeutic target for a variety of cancers, including colon cancer, stomach cancer, liver cancer, breast cancer, and lung cancer (2).
References :1. Meyerson, M. and Harlow, E. Identification of G1 kinase activity for cdk6, a novel cyclin D partner. Mol Cell Biol. 14(3):2077-2086, 1994.
2. Chen, G. et al. miR-93-5p suppresses ovarian cancer malignancy and negatively regulate CCND2 by binding to its 3’TUR region. Discov Oncol. 13(1):15, 2022.
Purity :Sample Purity. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Observed MW ~60 kDa.
Calculated MW ~59 kDa.
Product Sheets (By Lot #) :
Y4280-1.pdf Research Areas :Cancer, Cell Cycle, Cellular Stress